Complementary nuclear RNA's of murine sarcoma-leukemia virus complex in transformed cells.

نویسندگان

  • N Biswal
  • M Benyesh-Melnick
چکیده

The high molecular weight RNA (S(20,w) = 69S) of murine sarcomaleukemia virus (MSV-MLV) complex dissociated into 37S subunits when heated to 95 degrees C for 2 minutes. Specific annealing tests revealed that the viral RNA had complementarity with two heterogeneous RNA species from the nuclei of cells (78 A1) transformed by and chronically infected with this virus complex. The two nuclear RNA species that hybridized with the viral RNA had sedimentation constants ranging from 31-36S and 18-22S, respectively. There was no specific annealing between viral RNA and the cytoplasmic RNA of the 78 A1 cells. The detection of the complementary RNA strands of MSV-MLV suggests that this oncogenic virus complex may replicate in a fashion similar to that of the non-oncogenic RNA viruses, but may differ in that the complementary strands are found in the nucleus rather than in the cytoplasm of the transformed cells.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Selection of revertants of Kirsten sarcoma virus transformed nonproducer BALB-3T3 cells.

Revertants of Kirsten sarcoma virus transformed nonproducer BALB/3T3 cells (KA31 cells) were isolated after exposing the transformed cells to 5-fluorodeoxyuridine at high cell density, or when suspended in methylcellulose. Revertants were also isolated by treating KA31 cells with the lectin, concanavalin A, which is manyfold more toxic to transformed cells than for normal cells. The revertants ...

متن کامل

Multinucleation in the presence of cytochalasin B by RNA tumor virus-transformed cells.

RNA tumor virus-transformed cell cultures derived from rat, mouse, hamster, and mink were examined for their response to cytochalasin B (CB), and the expression of this marker was correlated with growth in soft agar and tumorigenicity in vivo. Continuous cell lines transformed and chronically infected with Moloney murine sarcoma-leukemia virus (M-MSV-MuLV) or Kirsten murine sarcoma-leukemia vir...

متن کامل

Activation of the murine sarcoma virus genome after infection with the murine leukemia virus as determined by cell agglutination.

Non-virus-producing NIH/3T3 cells transformed by the murine sarcoma virus are agglutinated by conconavalin A to the same low level as normal NIH/3T3 cells. Infection with the murine leukemia virus greatly increases the agglutination of transformed cells but not that of normal cells. These data suggest that the morphological expression of cell transformation and the surface alterations associate...

متن کامل

Demonstration of a cell-surface antigen associated with murine sarcoma virus by immunoelectron microscopy.

Cells transformed by murine sarcoma virus have been examined for the presence of a new virus-associated cell-surface antigen by immunoelectron microscopy. A common antigen has been detected on the surface of nonproductively transformed cells that were induced by two different strains of murine sarcoma virus, Kirsten and Moloney. This antigen shows crossreaction with cell lines transformed by mu...

متن کامل

Separation of sarcoma virus-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus.

We have studied the nucleic acid sequences in nonproducer cells transformed by Moloney sarcoma virus or Abelson leukemia virus (two types of replication-defective, RNA-containing, viruses isolated by passage of Moloney leukemia virus in BALB/c mice). DNA probes from the Moloney leukemia in virus detect RNA in both Abelson virus-transformed nonproducer cells and Moloney sarcoma virus-transformed...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 64 4  شماره 

صفحات  -

تاریخ انتشار 1969